Thermo Fisher Scientific IDH1 Polyclonal Antibody
Thermo Fisher Scientific의 IDH1 Polyclonal Antibody는 인간, 마우스, 랫트 시료에 반응하며 Western blot, ICC/IF, ELISA, IP 등에 사용 가능. 높은 특이성과 재현성을 제공하며 세포질 및 퍼옥시좀 내 IDH1 단백질 검출에 적합. 연구용으로만 사용.
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Thermo Fisher Scientific IDH1 Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5–4 µg antibody for 200–400 µg extracts |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing amino acids 1–414 of human IDH1 (NP_005887.2) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.83 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2805363 |
Product Specific Information
Immunogen sequence:
MSKKISGGSV VEMQGDEMTR IIWELIKEKL IFPYVELDLH SYDLGIENRD ATNDQVTKDA AEAIKKHNVG VKCATITPDE KRVEEFKLKQ MWKSPNGTIR NILGGTVFRE AIICKNIPRL VSGWVKPIII GRHAYGDQYR ATDFVVPGPG KVEITYTPSD GTQKVTYLVH NFEEGGGVAM GMYNQDKSIE DFAHSSFQMA LSKGWPLYLS TKNTILKKYD GRFKDIFQEI YDKQYKSQFE AQKIWYEHRL IDDMVAQAMK SEGGFIWACK NYDGDVQSDS VAQGYGSLGM MTSVLVCPDG KTVEAEAAHG TVTRHYRMYQ KGQETSTNPI ASIFAWTRGL AHRAKLDNNK ELAFFANALE EVSIETIEAG FMTKDLAACI KGLPNVQRSD YLNTFEFMDK LGENLKIKLA QAKL
Positive Samples: 293T, HepG2, HeLa, U-87MG, Mouse liver, Rat liver
Cellular Location: Cytoplasm, Peroxisome
Target Information
Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses: NAD(+)-dependent and NADP(+)-dependent.
Five isocitrate dehydrogenases have been reported:
- Three NAD(+)-dependent isocitrate dehydrogenases localized to the mitochondrial matrix
- Two NADP(+)-dependent isocitrate dehydrogenases (one mitochondrial, one cytosolic)
The NADP(+)-dependent cytoplasmic isozyme encoded by this gene is found in cytoplasm and peroxisomes, containing a PTS-1 peroxisomal targeting signal sequence.
This enzyme contributes to NADPH regeneration for intraperoxisomal reductions and cytoplasmic NADPH production.
Safety and Regulatory Information
⚠ WARNING: This product can expose you to chemicals including mercury, known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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