Thermo Fisher Scientific Survivin Recombinant Rabbit Monoclonal Antibody (1U9K8)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:5,000

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

1U9K8

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-142 of human Survivin (NP_001159.2). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Survivin,uniProtId:O15392-1,ncbiNodeId:9606,antigenRange:1-142,antigenLength:142,antigenImageFileName:MA5-51393_Survivin_O15392-1_Rabbit.svg,antigenImageFileNamePDP:MA5-51393_Survivin_O15392-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 0.05% BSA, 50% glycerol

Contains

0.05% ProClin 300

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_3093088

Product Specific Information

Immunogen sequence: MGAPTLPPAW QPFLKDHRIS TFKNWPFLEG CACTPERMAE AGFIHCPTEN EPDLAQCFFC FKELEGWEPD DDPIEEHKKH SSGCAFLSVK KQFEELTLGE FLKLDRERAK NKIAKETNNK KKEFEETAEK VRRAIEQLAA MD

Target Information

Survivin (IAP4) is the smallest member of the Inhibitors of Apoptosis Protein (IAP) gene family. The IAPs are involved in multiple cell functions such as cell signaling, cell division, metabolism, and exhibits differential expression in nearly all human cancers, but not in most normal tissues. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but the Survivin gene encodes proteins with only a single BIR domain. The encoded proteins also lack a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors yet low in adult tissues. Antisense transcripts are involved in the regulation of survivins gene expression. Survivin is expressed in the G2/M phase of the cell cycle in a cycle-regulated manner. At the beginning of mitosis, survivin associates with microtubules of the mitotic spindle in a specific and saturable reaction that is regulated by microtubule dynamics. Disruption of survivin-microtubule interactions results in loss of survivins anti-apoptosis function and increased caspase-3 activity, a mechanism involved in cell death, during mitosis. Survivin may counteract a default induction of apoptosis in G2/M phase. Survivin is also abundantly expressed in brain tissues (astrocytes and some neurons) of adult rats following traumatic brain injury. Survivin has been found co-expressed with NeuN (mature neuronal marker) and PCNA (a cell cycle protein). Survivin might affect regulation of neural cell proliferative responses after brain injury. At least four transcript variants encoding distinct isoforms have been found for this gene, but the full-length natures of only three of them have been determined. The overexpression of survivin in cancer may overcome this apoptotic checkpoint and favor aberrant progression of transformed cells through mitosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.