Thermo Fisher Scientific PRRX1 Monoclonal Antibody (OTI2E8), TrueMAB
PRRX1 단백질을 표적하는 Mouse IgG2a 단클론 항체로, Western blot에 최적화됨. 인간에 반응하며, 무보존제 PBS 버퍼에 동결건조 형태로 제공. 재구성 및 결합 실험에 적합한 고순도 항체.
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Applications
- Western Blot (WB): Tested dilution 1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2E8 |
| Immunogen | Full length human recombinant protein of PRRX1 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
The homeobox DNA-binding domain is a conserved 60 amino acid motif that binds DNA via a helix-turn-helix structure, playing a key role in transcriptional regulation and gene expression control.
PRRX1 (paired related homeobox 1), also known as PMX1 or PHOX1, is a 245 amino acid nuclear protein containing one OAR domain and one homeobox domain. It acts as a transcriptional co-activator enhancing SRF-dependent gene expression and regulates creatine kinase-M transcriptional activity, contributing to mesodermal muscle type establishment.
PRRX1 exists as two alternatively spliced isoforms, PMX1-A and PMX1-B.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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