Thermo Fisher Scientific CD43 Monoclonal Antibody (eBio84-3C1 (84-3C1)), FITC, eBioscience

Thermo Fisher Scientific CD43 Monoclonal Antibody (eBio84-3C1 (84-3C1)), FITC, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.25 µg)/test
Publications: View 9 publications

Product Specifications

Available Formats

• Unconjugated
• APC
• NovaFluor Blue 510
• NovaFluor Blue 585
• NovaFluor Blue 690
• NovaFluor Yellow 570
• PE
• PE-Cyanine7
• Request custom conjugation

Product Specific Information

The monoclonal antibody eBio84-3C1 reacts with CD43 (leukosialin, galactoglycoprotein, sialophorin). CD43 is a sialomucin with both adhesive and anti-adhesive functions. It is expressed on most leukocytes except resting B lymphocytes, and its surface expression decreases upon activation due to proteolytic processing. CD43 participates in activation of T cells, B cells, NK cells, and monocytes. The counter-receptor for CD43 is CD169/SIGLEC-1, expressed on macrophages.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested on peripheral blood mononuclear cells at 5 µL (0.25 µg) per test.
A test is defined as the amount of antibody staining a cell sample in 100 µL final volume. Cell number may vary (10⁵–10⁸ cells/test).
Excitation: 488 nm
Emission: 520 nm
Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD43 (leukosialin, sialophorin) is a transmembrane mucin-like protein with high negative charge, expressed on most hematopoietic cells. It forms a repulsive barrier interfering with adhesion but can also promote leukocyte aggregation. Through interaction with actin-binding proteins ezrin and moesin, CD43 regulates T-cell morphology and cell-cell interactions during lymphocyte trafficking. CD43 signaling enhances LFA-1 adhesiveness while counteracting other receptor-induced LFA-1 activation. Expression of CD43 induces tumor suppressor p53, but in p53/ARF-deficient cases, it promotes tumor proliferation. CD43 dysfunction is associated with Wiscott-Aldrich Syndrome and Adenoid Basal Cell Carcinoma.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.

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