Thermo Fisher Scientific CD43 Monoclonal Antibody (eBioR2/60), FITC, eBioscience

Thermo Fisher Scientific CD43 Monoclonal Antibody (eBioR2/60), FITC, eBioscience

Applications

• Flow Cytometry (Flow)
  Tested dilution: 0.125 µg/test
  View 22 publications

Product Specifications

Additional Formats

• Unconjugated
• Biotin
• PE
• Request custom conjugation

Product Specific Information

The eBioR2/60 monoclonal antibody reacts with mouse CD43, also known as leukosialin and sialophorin.
CD43 has glycoforms of approximately 115 and 130 kDa, and is expressed by early B cell subsets in bone marrow, thymocytes, mature T cells, NK cells, myeloid lineage cells, and platelets.
CD43 binds to CD54 and plays a role in adhesion and B cell survival.

While the S7 and 1B11 monoclonal antibody clones detect only certain isoforms of CD43, the R2/60 clone detects both isoforms.
R2/60 stains the majority of CD4+ and CD8+ splenocytes, as well as a significant population of CD19+ splenocytes.
This suggests that R2/60 recognizes an epitope shared by both isoforms of CD43.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Mouse splenocytes (≤ 0.125 µg/test, 100 µL final volume)
  • Recommended titration for optimal performance
  • Excitation: 488 nm; Emission: 520 nm; Laser: Blue
  • Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD43 (leukosialin, sialophorin) is a transmembrane mucin-like protein with high negative charge, expressed on most hematopoietic cells.
CD43 contributes to a repulsive barrier that interferes with cellular adhesion but can also promote leukocyte aggregation.
Through interactions with actin-binding proteins (ezrin, moesin), CD43 regulates T-cell morphology and cell-cell interactions during lymphocyte traffic.
CD43 signaling modulates LFA-1 adhesiveness and can counteract LFA-1 induction via other receptors.
Expression of CD43 can induce p53 tumor suppressor activity, but in p53/ARF deficiency, CD43 may promote tumor proliferation.
Associated diseases include Wiscott-Aldrich Syndrome and Adenoid Basal Cell Carcinoma.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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