Thermo Fisher Scientific Ubiquitin Monoclonal Antibody (5B9.B3)

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

-

Immunocytochemistry (ICC/IF)

10 µg/mL

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

All, Bovine, Human, Mouse, Rat

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

5B9.B3

Immunogen

Native bovine ubiquitin conjugated to KLH if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Ubiquitin,uniProtId:P0CG53-1,ncbiNodeId:9913,antigenRange:1-305,antigenLength:305,antigenImageFileName:11023-100UG_Ubiquitin_P0CG53-1_House_mouse.svg,antigenImageFileNamePDP:11023-100UG_Ubiquitin_P0CG53-1_House_mouse_PDP.jpeg,sortOrder:7}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5-1.0 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Target Information

Ubiquitin, a highly conserved protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome, is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein S27a at the C terminus. When expressed in yeast, the protein is post-translationally processed, generating free ubiquitin monomer and ribosomal protein S27a. Ribosomal protein S27a is a component of the 40S subunit of the ribosome and belongs to the S27AE family of ribosomal proteins. It contains C4-type zinc finger domains and is located in the cytoplasm. Pseudogenes derived from this gene are present in the genome. As with ribosomal protein S27a, ribosomal protein L40 is also synthesized as a fusion protein with ubiquitin; similarly, ribosomal protein S30 is synthesized as a fusion protein with the ubiquitin-like protein fubi. Multiple alternatively spliced transcript variants that encode the same proteins have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.