Thermo Fisher Scientific CD4 Monoclonal Antibody (RM4-5), Brilliant Violet 480, eBioscience

Thermo Fisher Scientific CD4 Monoclonal Antibody (RM4-5), Brilliant Violet 480, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 1.0 µg/test

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 532
• Alexa Fluor 561
• Alexa Fluor 647
• Alexa Fluor 660
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395, 496, 563, 615, 661, 737, 805
• Brilliant Violet 421, 605, 650, 711, 786
• eFluor 450, 506, 570, 615
• FITC
• NovaFluor Blue 585, 610-70S, 660-120S, 760
• NovaFluor Yellow 590, 610, 660
• Pacific Orange
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Request custom conjugation

Product Specific Information

The RM4-5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by most thymocytes, subsets of mature T cells, and dendritic cells. CD4 binds to MHC class II on antigen-presenting cells and plays a key role in T cell development and function. In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail. Binding of RM4-5 is blocked by GK1.5.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested by flow cytometric analysis of mouse splenocytes at ≤1.0 µg/test. A test is defined as staining a cell sample in 100 µL volume, with cell counts ranging from 10⁵–10⁸ cells/test. Antibody titration is recommended for optimal results.

Brilliant Violet™ 480 (BV480):

• Emits at 479 nm
• Intended for use with cytometers equipped with a violet (405 nm) laser
• Ensure instrument compatibility before use

When using multiple polymer dye-conjugated antibodies (e.g., Super Bright, Brilliant Violet™, Brilliant Ultra Violet™), use Super Bright Complete Staining Buffer (Product # SB-4401) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific interactions.

Fixation Compatibility: BV480 is not compatible with methanol-based fixation.
Excitation: 440 nm
Emission: 479 nm
Laser: Violet laser

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

CD4 antigen is involved in MHC class II recognition and serves as a co-receptor for HIV. It is primarily expressed in T helper cells but also found in monocytes, macrophages, and dendritic cells. CD4 expression occurs in thymus, lymph nodes, tonsils, spleen, and certain non-lymphoid tissues including brain and gut. It mediates early T-cell activation and may contribute to neuronal damage in immune-mediated CNS diseases. Multiple alternatively spliced transcripts have been identified (RefSeq, July 2017).

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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