Thermo Fisher Scientific COX5B Monoclonal Antibody (16H12H9)

Applications

Western Blot (WB)

• Tested Dilution: 1.0 µg/mL
• View 4 publications

Flow Cytometry (Flow)

• Tested Dilution: 1 µg per 10^6 cells

Product Specifications

Product Specific Information

Positive control: Isolated mitochondria from Human heart, Bovine heart, Rat heart, and Mouse heart

Target Information

The cytochrome c oxidase (COX) family of proteins functions as the final electron donor in the respiratory chain to drive a proton gradient across the inner mitochondrial membrane, ultimately resulting in the production of water.
The mammalian COX apoenzyme is a dimer, with each monomer consisting of 13 subunits, some mitochondrial and some nuclear.
Found in the inner mitochondrial membrane, COX5 is the heme A-containing chain of the oxidase family that converts one molecule of oxygen and four molecules of hydrogen to two molecules of water.
Two isoforms of COX5 exist, COX5a and COX5b. When oxygen levels are high, transcription of COX5a (aerobic isoform) increases; when oxygen levels are low, COX5b (hypoxic isoform) transcription increases to maximize turnover rate of the COX apoenzyme.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.