Thermo Fisher Scientific COX5B Monoclonal Antibody (16H12H9)

Applications

Tested Dilution

Publications

Western Blot (WB)

1.0 µg/mL

View 4 publications 4 publications

Flow Cytometry (Flow)

1 µg per 10^6 cells

-

Product Specifications

Species Reactivity

Bovine, Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

16H12H9

Immunogen

Bovine Complex IV (native holoenzyme protein, purified from heart) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:COX5B,uniProtId:P00428-1,ncbiNodeId:9913,antigenRange:1-129,antigenLength:129,antigenImageFileName:459110_COX5B_P00428-1_House_mouse.svg,antigenImageFileNamePDP:459110_COX5B_P00428-1_House_mouse_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Ammonium sulfate precipitation

Storage buffer

HEPES buffered saline

Contains

0.02% sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Wet ice

RRID

AB_10376329

Product Specific Information

Positive control: Isolated mitochondria from Human heart, Bovine heart, Rat heart, and Mouse heart

Target Information

The cytochrome c oxidase (COX) family of proteins function as the final electron donor in the respiratory chain to drive a proton gradient across the inner mitochondrial membrane, ultimately resulting in the production of water. The mammalian COX apoenzyme is a dimer, with each monomer consisting of 13 subunits, some of which are mitochondrial and some of which are nuclear. Found in the inner mitochondrial membrane, COX5 is the heme A-containing chain of the oxidase family that converts one molecule of oxygen and four molecules of hydrogen to two molecules of water. Two isoforms of COX5 exist, COX5a and COX5b. When oxygen levels within the cell are high, transcription of COX5a (the aerobic isoform) is up-regulated as the rate of cellular respiration increases. Conversely, when oxygen levels are low, COX5b (the hypoxic isoform) transcription increases and functions to maximize the turnover rate of the COX apoenzyme.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.