Thermo Fisher Scientific COX5A Monoclonal Antibody (6E9B12D5)

Applications

Tested Dilution

Publications

Western Blot (WB)

1.0 µg/mL

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Immunohistochemistry (IHC)

-

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Immunocytochemistry (ICC/IF)

5 µg/mL

-

Flow Cytometry (Flow)

1 µg/mL

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Product Specifications

Species Reactivity

Bovine, Human, Mouse, Rat

Published species

Human

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

6E9B12D5

Immunogen

Bovine COX5A

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Ammonium sulfate precipitation

Storage buffer

HEPES buffered saline

Contains

0.02% sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Wet ice

RRID

AB_10376328

Product Specific Information

Positive control: Isolated mitochondria from Human heart, Bovine heart, Rat heart, Mouse heart, and HepG2, Human embryonic lung-derived fibroblasts (strain MRC5), HeLa cells.

Target Information

Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer of proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit Va of the human mitochondrial respiratory chain enzyme. A pseudogene COX5AP1 has been found in chromosome 14q22.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.