Thermo Fisher Scientific HA tag Recombinant Rabbit Monoclonal Antibody (109B2), MonoRab

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.2 µg/mL

-

Immunocytochemistry (ICC/IF)

0.5-2 µg/mL

-

ELISA (ELISA)

0.05-0.2 µg/mL

-

Product Specifications

Species Reactivity

Tag

Host/Isotype

Rabbit / IgG

Expression System

Mammalian cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

109B2

Immunogen

a synthetic peptide containing the influenza hemagglutinin epitope (YPYDVPDYA). conjugated to KLH

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• Biotin
• Request custom conjugation

Form

Lyophilized

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.02% sodium azide

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.

The rabbit immune system generates antibody diversity and optimizes affinity. GenScript utilizes MonoRab™ technology to generate high affinity and specific monoclonal rabbit antibodies. The HA tag is composed of several amino acids containing YPYDVPDYA and does not appear to interfere with the bioactivity or the biodistribution of recombinant proteins. GenScript MonoRab™ HA tag Antibody (109B2), mAb, Rabbit is specific to HA tags placed at the N-terminal, C-terminal, and internal regions of fusion proteins. This antibody can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify and detect HA-tagged fusion proteins in bacteria, insect cells, and mammalian cells.

Target Information

HA Tag (hemagglutinin tag) is an epitope tag that provides a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. HA (hemagglutinin) is a surface glycoprotein derived from influenza hemagglutinin, and is commonly expressed as a tag in conjunction with proteins to aid in identification and purification of the tagged protein for functional analysis. HA tag antibodies can be used to facilitate protein detection or co-immunopreciptation of tagged proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.