Invitrogen™

pLenti6.2-GW/EmGFP Expression Control Vector

The Vivid Colors™ pLenti6.2-GW⁄EmGFP Expression Control Vector is a ViraPower™ positive control lentiviral vector containing Emerald Green Fluorescent Protein (EmGFP).자세히 알아보기

The Vivid Colors™ pLenti6.2-GW⁄EmGFP Expression Control Vector is a ViraPower™ positive control lentiviral vector containing Emerald Green Fluorescent Protein (EmGFP). It is designed for use with the ViraPower™ Lentiviral Expression Systems as a positive control to enable the detection of EmGFP fluorescence following transfection in 293FT cells, as a titer control to produce an EmGFP-expressing lentivirus stock and as a transduction control following transduction in both dividing and non-dividing mammalian cells. The vector has the CMV promoter for driving constitutive expression of EmGFP and the PGK promoter for driving long-term, persistent expression of the Blasticidin stable selection marker. This is not a cloning vector.

Advantages
• Optimization of viral transduction efficiency
• Optimization of 293FT Transfection
• 2 day titer of functional virus using EmGFP

Key Features
• Human cytomegalovirus (CMV) immediate early promoter to control high-level expression of the EmGFP
• PKG Promoter for expression of Blasticidin selection marker

Kit Includes
pLenti6.2-GW⁄EmGFP Vector

Related SKUs
• 293FT Cell Line (R70007; R70007)
• ViraPower™ Lentiviral Support Kit (K4970-00)
• ViraPower™ Lentiviral Packaging Mix (K4975-00)
• Lipofectamine™ 2000 (11668-019; 11668-027)

For research use only. Not intended for any therapeutic or diagnostic use.

사양

구성 또는 유도성 시스템Constitutive

배달 유형Lentiviral

프로모터CMV

제품 유형Lentiviral Expression Vector

리포터 유전자GFP (EmGFP)

선택 제제(진핵)Blasticidin

선택 마커 프로모터PGK Promoter

단백질 태그Untagged

클로닝 방법Gateway

수량1 Ea.

벡터pLenti

제품라인Gateway™, ViraPower™, Vivid Colors™

용도(애플리케이션)Reporter Assays, Viral Expression

Unit Size1 each