Thermo Fisher Scientific Rtf1 Polyclonal Antibody
Rtf1 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. Western blot, IHC, IP 등 다양한 응용에 적합. 항원 친화 크로마토그래피로 정제되어 높은 특이성과 재현성 제공. 연구용으로만 사용.
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Thermo Fisher Scientific Rtf1 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | C-terminus of Human Restores TBP (TATA box-binding protein) Function-1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | TBS, pH 7.0–8.0, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Target Information
Three isoforms of serine/threonine kinases, designated alphaPAK p68, betaPAK p65, and gammaPAK p62, exhibit high sequence homology with the S. cerevisiae kinase Ste20, involved in pheromone signaling. The alpha, beta, and gammaPAK isoforms complex specifically with Rac1 and Cdc42 in their active GTP-bound state, inhibiting their intrinsic GTPase activity and leading to autophosphorylation.
There are eight sites of autophosphorylation on gammaPAK, including Ser19, Ser141, and Thr402, and phosphorylation of Ser141 and Thr402 correlates with gammaPAK activation. Once phosphorylated, the PAK isoforms dissociate from Rac/Cdc42 to interact with downstream substrates.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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