Thermo Fisher Scientific CD209 (DC-SIGN) Monoclonal Antibody (eB-h209), Biotin, eBioscience

Applications

Tested Application

• Flow Cytometry (Flow)

Tested Dilution

• 1 µg/test

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Product Specifications

Additional Formats Available

• Unconjugated
• APC
• FITC
• PE
• PE-Cyanine7
• PerCP-Cyanine5.5
• Request custom conjugation

Product Specific Information

The eB-h209 monoclonal antibody reacts with human CD209 (DC-SIGN), a 44 kDa type II transmembrane protein containing a C-type lectin binding domain. DC-SIGN binds ICAM-3, ICAM-2, and HIV virus, and is preferentially expressed on human dendritic cells. It is suggested to function as a receptor for HIV capture and transmission to T cells, supporting the primary immune response. The antibody was developed against a C-terminal peptide of human DC-SIGN.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested by flow cytometry on cultured human dendritic and peripheral blood cells
  • Recommended ≤ 1 µg per test (100 µL final volume)
  • Cell number: 10⁵–10⁸ cells/test
  • Optimize titration for best performance
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

The CD209 gene encodes a transmembrane receptor (DC-SIGN) expressed on dendritic cells and macrophages. It plays a key role in the innate immune system by recognizing a wide range of pathogens including parasites and viruses. The protein has three main domains:

• N-terminal transmembrane domain
• Tandem-repeat neck domain
• C-type lectin carbohydrate recognition domain

The extracellular region functions as both a pathogen recognition and cell adhesion receptor. The neck domain allows homo-oligomerization, increasing binding avidity to multivalent ligands. Variations in the neck domain repeat number can affect ligand binding. CD209 is closely related to L-SIGN (GeneID 10332), differing in ligand-binding properties and tissue distribution. Alternative splicing results in multiple variants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.