Thermo Fisher Scientific CD201 (EPCR) Monoclonal Antibody (eBio1560 (1560)), Biotin, eBioscience
Mouse CD201(EPCR)을 인식하는 Biotin-conjugated monoclonal antibody. Flow cytometry 및 IHC에 적합. 혈액응고 및 염증 조절 관련 연구에 활용 가능. 0.5 mg/mL 농도, 4°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | Assay-Dependent | View 3 publications |
| Immunocytochemistry (ICC/IF) | - | View 9 publications |
| Flow Cytometry (Flow) | 1 µg/test | View 8 publications |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse, Non-human primate |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), Biotin, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBio1560 (1560) |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_657694 |
Available Formats
Product Specific Information
The eBio1560 monoclonal antibody reacts with mouse Endothelial Protein C Receptor (EPCR, CD201), a 25 kDa type I transmembrane protein expressed on endothelial cells. EPCR shows sequence and structural homology with the MHC class I/CD1 family of proteins. It acts as a ligand for Protein C and plays a critical role in augmenting Protein C activation by the thrombin-thrombomodulin complex, regulating blood coagulation and inflammation. Deletion of EPCR results in embryonic lethality, partly due to placental thrombosis.
EPCR expression marks bone marrow cells capable of hematopoietic reconstitution comparable to hematopoietic stem cells isolated via conventional methods. The eBio1560 antibody can be used for detecting these hematopoietic stem cells, while the eBiomRCR-16 monoclonal antibody is recommended for endothelial CD201 detection.
Applications Reported: Flow cytometric analysis and immunohistochemical staining.
Applications Tested: Tested by flow cytometric analysis of bEnd.3 cells. Use ≤1 µg per test (100 µL final volume). Optimize antibody titration for best performance, particularly for endothelial cells with high CD201 expression.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
The gene encodes a receptor for activated protein C, a serine protease involved in blood coagulation. The protein is an N-glycosylated type I membrane receptor that enhances protein C activation. Mutations are associated with venous thromboembolism, myocardial infarction, and late fetal loss during pregnancy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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배송/결제/교환/반품 안내
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