Thermo Fisher Scientific CD201 (EPCR) Monoclonal Antibody (eBio1560 (1560)), Biotin, eBioscience

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

Assay-Dependent

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

-

View 9 publications 9 publications

Flow Cytometry (Flow)

1 µg/test

View 8 publications 8 publications

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse, Non-human primate

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), Biotin, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBio1560 (1560)

Conjugate

Biotin Biotin Biotin

View additional formats

• APC
• APC-eFluor 780
• Functional Grade
• PE
• PerCP-eFluor 710
• Request custom conjugation

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_657694

Product Specific Information

Description: The eBio1560 monoclonal antibody reacts with mouse Endothelial Protein C Receptor (EPCR, CD201), a 25 kDa Type 1 transmembrane protein expressed on endothelial cells. EPCR exhibits sequence and structural homology with the MHC class I/CD1 family of proteins. EPCR is a ligand for Protein C and plays an important role in augmenting Protein C activation by the thrombin-thrombomodulin complex and in regulating blood coagulation and inflammation. Deletion of EPCR results in embryonic lethality, at least partically due to placental thrombosis.

Recently, it was demonstrated that EPCR expression identified cells in the bone marrow that are capable of hematopoietic reconstitution activity comparable to hematopoietic stem cells isolated with conventional methods. The eBio1560 monoclonal antibody can be used for the detection of these hematopoietic stem cells, however the eBiomRCR-16 monoclonal antibody should only be used for the detection of CD201 on endothelial cells.

Applications Reported: This eBio1560 (1560) antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining.

Applications Tested: This eBio1560 (1560) antibody has been tested by flow cytometric analysis of bEnd.3 cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest, particµLarly when analyzing expression on endothelial cells where CD201 expression is very high.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The protein encoded by this gene is a receptor for activated protein C, a serine protease activated by and involved in the blood coagulation pathway. The encoded protein is an N-glycosylated type I membrane protein that enhances the activation of protein C. Mutations in this gene have been associated with venous thromboembolism and myocardial infarction, as well as with late fetal loss during pregnancy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.