Thermo Fisher Scientific Rabbit anti-Human Ig (Light chain) Recombinant Secondary Antibody
Thermo Fisher Scientific의 재조합 토끼 단클론 항체로, 인간 Ig 경쇄를 인식하는 2차 항체입니다. Western blot, IHC, ICC, Flow cytometry, ELISA 등 다양한 응용에 적합합니다. 높은 특이성과 일관된 로트 간 품질을 제공합니다. 연구용으로만 사용 가능합니다.
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Applications 및 권장 희석 배수
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,500–1:5,000 |
| Immunohistochemistry (IHC) | 0.5–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 0.5–2 µg/mL |
| Flow Cytometry (Flow) | 0.5–2 µg/mL |
| ELISA | 0.1–0.5 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Secondary Antibody |
| Clone | RM129 |
| Immunogen | Human IgG |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with 1% BSA, 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2744981 |
| Target | Ig (Light chain) |
| Antibody Form | Whole Antibody |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems are developed by cloning the specific antibody DNA sequences from immunoreactive rabbits, followed by screening to select the best clones for production.
Advantages include:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to the larger rabbit immune repertoire
Target Information
Anti-Human secondary antibodies are affinity-purified and exhibit well-characterized specificity for human immunoglobulins. They are useful for detection, sorting, or purification of target molecules.
Key features:
- Compatible with multiple detection systems (HRP, AP, fluorescence)
- Provide signal amplification for improved sensitivity
- Generated by immunizing host animals with pooled immunoglobulins
- Can be further purified or modified (e.g., immunoaffinity chromatography, fragmentation, conjugation)
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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