Thermo Fisher Scientific Rabbit anti-Human IgG3 Recombinant Secondary Antibody, Biotin
Rabbit에서 유래한 재조합 단클론 항-Human IgG3 2차항체로, Biotin으로 표지되어 다양한 검출 시스템에 활용 가능. IHC, ICC/IF, ELISA에 적합하며, 높은 특이성과 일관된 로트 간 재현성을 제공. 단백질 A 정제 및 동물 유래 성분 무첨가 포뮬레이션.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (IHC) | 2–5 µg/mL |
| Immunocytochemistry (ICC/IF) | 2–5 µg/mL |
| ELISA | 50–200 ng/well |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Secondary Antibody |
| Clone | RM119 |
| Immunogen | Human IgG3 |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with 1% BSA, 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2744978 |
| Target | IgG3 |
| Antibody Form | Whole Antibody |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages include:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broad immunoreactivity due to the large rabbit immune repertoire
Target Information
Anti-Human secondary antibodies are affinity-purified with well-characterized specificity for human immunoglobulins. They are useful for detection, sorting, or purification of target molecules.
Key features:
- Compatible with multiple detection systems (e.g., HRP, AP, fluorescence)
- Provide signal amplification for greater sensitivity
- Commonly generated by immunizing host animals with pooled immunoglobulins from the target species
- Can be further purified or modified (e.g., immunoaffinity chromatography, fragmentation, label conjugation) for high specificity
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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