Thermo Fisher Scientific Rabbit anti-Human IgG4 Recombinant Secondary Antibody, Biotin
Rabbit 유래 재조합 단클론 항체로 Human IgG4 검출용 Biotin 결합 2차 항체. Western blot, Flow cytometry, ELISA에 적합. 높은 특이도와 재현성 제공. 단백질 A 정제 및 동물 유래 성분 없음. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.5–2 µg/mL |
| Flow Cytometry (Flow) | 1–4 µg/mL |
| ELISA | 50–200 ng/well (Capture), 0.05–0.2 µg/mL (Detect) |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Secondary Antibody |
| Clone | RM120 |
| Immunogen | Peptide corresponding to the hinge region of Human IgG4 |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with 1% BSA, 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2744979 |
| Target | IgG4 |
| Antibody Form | Whole Antibody |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems are developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select optimal candidates for production.
Advantages of recombinant rabbit monoclonal antibodies include:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broad immunoreactivity due to the extensive rabbit immune repertoire
Target Information
Anti-Human secondary antibodies are affinity-purified with well-characterized specificity for human immunoglobulins. They are used for detection, sorting, or purification of the target.
Secondary antibodies provide:
- Versatility across detection systems (HRP, AP, fluorescence)
- Increased sensitivity via signal amplification
- High specificity through immunoaffinity purification and conjugation modifications
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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