Thermo Fisher Scientific Parvalbumin Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1 µg/mL

View 4 publications 4 publications

Immunohistochemistry (IHC)

-

View 22 publications 22 publications

Immunohistochemistry (Paraffin) (IHC (P))

1 µg/mL

View 3 publications 3 publications

Immunohistochemistry - Free Floating (IHC (Free))

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100-1:200

View 5 publications 5 publications

ELISA (ELISA)

Assay-dependent

-

Immunoprecipitation (IP)

5 µg/mL

-

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Rat

Published species

Human, Mouse, Rat, Sea urchin, Zebrafish

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Purified parvalbumin from rat skeletal muscle. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Parvalbumin,uniProtId:P02625-1,ncbiNodeId:10114,antigenRange:1-110,antigenLength:110,antigenImageFileName:PA1-933_Parvalbumin_P02625-1_Rabbit.svg,antigenImageFileNamePDP:PA1-933_Parvalbumin_P02625-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.1M sodium phosphate, pH 7.0, with 20mg/mL BSA

Contains

0.1% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2173898

Product Specific Information

PA1-933 detects parvalbumin protein in human and rat samples.

PA1-933 has successfully been used in Western blot, ELISA, immunoprecipitation and immunohistochemical procedures. By Western blot, this antibody detects a 12 kDa protein representing parvalbumin from rat cerebellum.

Parvalbumin protein is relatively small and, therefore, it is recommended that the electrophoresis be performed using tricine-SDS-PAGE gels and transferred to a nylon membrane.

The PA1-933 immunizing protein corresponds to purified parvalbumin from rat skeletal muscle.

Reconstitute in 100 µL PBS to create a stock of 1 mg/mL.

Target Information

Parvalbumin (PV) is a calcium binding protein expressed in specific muscle fibers and fast-firing neurons. PV consists of a single, unbranched chain of linked amino acids and belongs to a larger group of EF hand proteins. Studies have demonstrated that parvalbumin acts in the decay of calcium in the contraction/ relaxation cycle of fast twitch muscles. This data has shown a positive correlation between the rate of relaxation and the concentration of parvalbumin. Parvalbumin is also expressed in a specific population of GABAergic interneurones which are thought to play a role in maintaining the balance between excitation and inhibition in the cortex as well as the hippocampus. In amyotrophic lateral sclerosis (ALS) patents, parvalbumin immunoreactivity is specifically absent from neuron populations lost early in ALS.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.