Thermo Fisher Scientific Calprotectin Monoclonal Antibody (MAC387)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:200

-

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:200

-

Immunocytochemistry (ICC/IF)

1:250

-

Flow Cytometry (Flow)

1:50-1:100

View 1 publication 1 publication

Product Specifications

Species Reactivity

Baboon, Bovine, Dog, Horse, Cat, Guinea pig, Goat, Human, Mouse, Non-human primate, Rabbit, Rat

Published species

Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

MAC387

Immunogen

Human monocytes if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Calprotectin,uniProtId:P05109-1,ncbiNodeId:9606,antigenRange:1-93,antigenLength:93,antigenImageFileName:MA1-33972_Calprotectin_P05109-1_House_mouse.svg,antigenImageFileNamePDP:MA1-33972_Calprotectin_P05109-1_House_mouse_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1957578

Product Specific Information

For staining of paraffin sections, protein digestion pre-treatment (e.g. trypsin), 0.1% for 10 minutes or antigen retrieval using heat treatment is required.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

The S100A8/A9 heterocomplex, also called MRP-8/MRP-14 complex or calprotectin, comprises 60% of the cytoplasmic protein fraction of circulating polymorphonuclear granulocytes and is also found in monocytes, macrophages and ileal tissue eosinophils. Peripheral blood monocytes carry the antigen extra- and intracellularly, neutrophils only intracellularly. The S100A8/A9 complex has antibacterial, antifungal, immunomodulating and antiproliferative effects. Besides this it is a potent chemotactic factor for neutrophils. Plasma concentrations are elevated in diseases associated with increased neutrophil activity, like inflammatory bowel disease. Granulocytes terminate their existence after transmigration through the intestinal wall. Therefore calprotectin is also detectable in feces. Elevated levels of calprotectin have been observed in body fluids such as plasma, saliva, gingival crevicular fluid, stools, and synovial fluid during infection and inflammatory conditions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.