Thermo Fisher Scientific CD278 (ICOS) Monoclonal Antibody (ISA-3), PE-Cyanine7, eBioscience
Human ICOS(CD278)을 인식하는 ISA-3 단클론 항체로, PE-Cyanine7 형광체가 결합되어 있습니다. Flow Cytometry에 최적화되어 있으며, 활성화된 T세포의 공자극 분자 발현 분석에 적합합니다. 4°C 암소 보관, 동결 금지.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Flow Cytometry (Flow) | 5 µL (0.125 µg)/test | View 20 publications |
| Functional Assay (Functional) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine7, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | ISA-3 |
| Conjugate | PE-Cyanine7 |
| Excitation / Emission Max | 569 / 780 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1518755 |
Additional Available Formats
- Unconjugated
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 661
- eFluor 450
- FITC
- Functional Grade
- NovaFluor Red 660
- NovaFluor Red 710
- NovaFluor Yellow 700
- NovaFluor Yellow 730
- PE
- PE-Cyanine5.5
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Super Bright 780
- Request custom conjugation
Product Specific Information
The ISA-3 monoclonal antibody reacts with human ICOS (Inducible COStimulatory molecule), also known as H4, CRP-1, and AILIM.
ICOS is a T cell-specific activation molecule and a member of the CD28/CTLA-4 family. It plays a critical role in T cell activation and humoral immune responses, particularly in memory B cell and plasma cell generation.
ICOS binds to its ligand B7h/B7RP-1 on activated antigen-presenting cells and inflamed tissues. Plate-bound ISA-3 is costimulatory for T cells and induces IL-4, IL-5, and IL-10 production.
- Generated against human ICOS antigen
- Clone ISA-3 has the same reactivity as F44
- C398.4A (anti-mouse ICOS/H4) cross-reacts with human ICOS but binds a different epitope
- For brightest staining of activated human T cells, use Product #13-9948-82 or #12-9948-42
Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated for flow cytometry of stimulated human peripheral blood cells
Recommended Use: 5 µL (0.125 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test)
Light Sensitivity: Protect from light (photo-oxidation sensitive)
Fixation: Compatible with IC Fixation Buffer (Product #00-8222) or 1-step Fix/Lyse Solution (Product #00-5333)
Excitation / Emission: 488–561 nm / 775 nm
Laser Compatibility: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered
Target Information
ICOS (CD278) encodes a cell-surface receptor of the CD28/CTLA-4 family. It functions as a homodimer and is essential in cell-cell signaling, immune response regulation, and cell proliferation.
Mutations or dysregulation are associated with immunodeficiency disorders such as Common Variable Immunodeficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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