Thermo Fisher Scientific CD278 (ICOS) Monoclonal Antibody (ISA-3), PE-Cyanine7, eBioscience

Applications and Tested Dilutions

Product Specifications

Additional Available Formats

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 661
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Red 660
• NovaFluor Red 710
• NovaFluor Yellow 700
• NovaFluor Yellow 730
• PE
• PE-Cyanine5.5
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 780
• Request custom conjugation

Product Specific Information

The ISA-3 monoclonal antibody reacts with human ICOS (Inducible COStimulatory molecule), also known as H4, CRP-1, and AILIM.
ICOS is a T cell-specific activation molecule and a member of the CD28/CTLA-4 family. It plays a critical role in T cell activation and humoral immune responses, particularly in memory B cell and plasma cell generation.

ICOS binds to its ligand B7h/B7RP-1 on activated antigen-presenting cells and inflamed tissues. Plate-bound ISA-3 is costimulatory for T cells and induces IL-4, IL-5, and IL-10 production.

• Generated against human ICOS antigen
• Clone ISA-3 has the same reactivity as F44
• C398.4A (anti-mouse ICOS/H4) cross-reacts with human ICOS but binds a different epitope
• For brightest staining of activated human T cells, use Product #13-9948-82 or #12-9948-42

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated for flow cytometry of stimulated human peripheral blood cells
Recommended Use: 5 µL (0.125 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test)

Light Sensitivity: Protect from light (photo-oxidation sensitive)
Fixation: Compatible with IC Fixation Buffer (Product #00-8222) or 1-step Fix/Lyse Solution (Product #00-5333)
Excitation / Emission: 488–561 nm / 775 nm
Laser Compatibility: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered

Target Information

ICOS (CD278) encodes a cell-surface receptor of the CD28/CTLA-4 family. It functions as a homodimer and is essential in cell-cell signaling, immune response regulation, and cell proliferation.
Mutations or dysregulation are associated with immunodeficiency disorders such as Common Variable Immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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