
Thermo Fisher Scientific Alpha 1 Na+/K+ ATPase Polyclonal Antibody
Rabbit polyclonal antibody targeting Na⁺/K⁺ ATPase α1 subunit. Validated for WB, IHC-P, and IHC-F. High specificity for human, mouse, and rat. Lyophilized form, reconstitutable in DDW. For research use only.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:200 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:400 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | (C)RDKYEPAAVSEHGD, corresponding to amino acid residues 7–20 of rat ATP1A1, intracellular, N-terminus |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.8 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Reconstitution: Add 25 µL, 50 µL, or 0.2 mL of double-distilled water (DDW), depending on sample size.
The antibody ships lyophilized at room temperature and should be stored at -20°C upon arrival.
Reconstituted solution can be stored at 4°C for up to 1 week. For longer storage, aliquot and keep at -20°C.
Avoid repeated freeze-thaw cycles. Centrifuge all antibody preparations before use (10,000 × g, 5 min).
Target Information
Na⁺/K⁺-ATPase is a P-type cation transport ATPase responsible for maintaining the electrochemical gradients of Na⁺ and K⁺ ions across the plasma membrane. These gradients are critical for osmoregulation, sodium-coupled transport of molecules, and electrical excitability in nerve and muscle cells.
The enzyme consists of two subunits: a large catalytic α-subunit and a smaller glycoprotein β-subunit.
This antibody targets the α1 subunit encoded by the ATP1A1 gene, for which multiple transcript variants and isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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