Thermo Fisher Scientific WAVE1 Polyclonal Antibody
Thermo Fisher Scientific의 WAVE1 Polyclonal Antibody는 인간, 마우스, 랫트에 반응하는 Rabbit IgG 항체로, Western blot, IHC, ICC/IF에 사용 가능. 액상 형태로 항원 친화 크로마토그래피로 정제되었으며, 신경세포 내 actin 재구성 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of mouse WAVE1. The exact sequence is proprietary. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.73 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2736791 |
Product Specific Information
- Positive Control: Mouse brain, Rat brain
- Predicted Reactivity: Chimpanzee (100%), Bovine (100%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
WASP (Wiskott-Aldrich syndrome protein) and N-WASP are downstream effectors of Cdc42 involved in actin polymerization and cytoskeletal organization. The WASP family includes VASP and Mena, which accumulate at focal adhesions and regulate actin cytoskeleton dynamics. WAVE proteins, related to WASP family proteins, mediate actin reorganization downstream of Rho GTPases. WAVE1 and WAVE2 specifically regulate membrane ruffling by inducing actin filament clusters in response to Rac activation. These proteins cooperate with the Arp2/3 complex to promote actin filament formation. WAVE1 (also known as SCAR) is primarily expressed in the brain, while WAVE2 is broadly expressed, highest in leukocytes. WAVE3 forms a multiprotein complex linking receptor kinases and actin, all sharing a C-terminal verprolin homology domain for actin binding. This complex transduces signals related to cell shape, motility, and function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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