Thermo Fisher Scientific CD4 Monoclonal Antibody (GK1.5), PE-eFluor 610, eBioscience
마우스 CD4 분자에 특이적인 GK1.5 단클론 항체로, PE-eFluor 610 형광체로 표지되어 있습니다. 유세포분석(Flow Cytometry)에 적합하며, 0.25 µg/test로 사용 가능합니다. 488–561 nm 레이저로 여기되며 607 nm에서 방출합니다. 연구용으로만 사용하십시오.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Flow Cytometry (Flow) | 0.25 µg/test | View 165 publications |
| Functional Assay (Functional) | - | View 2 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Fish, Human |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), PE-eFluor™ 610, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | GK1.5 |
| Conjugate | PE-eFluor™ 610 |
| Excitation / Emission Max | 565 / 606 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2574518 |
Additional Available Formats
- Unconjugated
- Alexa Fluor 488, 532, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV, Violet, Yellow, Red, NovaFluor series
- PE, PE-Cyanine5, PE-Cyanine7
- PerCP-eFluor 710
- Super Bright series
- Request custom conjugation
Product Specific Information
The GK1.5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by thymocytes, subsets of mature T cells, and dendritic cells. CD4 binds to MHC class II on antigen-presenting cells and is critical for T cell development and function. In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail. Binding of GK1.5 is blocked by RM4-5.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on mouse splenocytes at ≤0.25 µg/test (100 µL final volume). Optimal titration recommended.
Fluorophore Information:
PE-eFluor® 610 is excited by 488–561 nm lasers and emits at 607 nm. Recommended detection filter: 610/20 band pass (equivalent to PE-Texas Red®). Protect from light due to photo-induced oxidation.
Fixation:
Samples can be stored up to 3 days at 4°C in the dark using IC Fixation Buffer (Product #00-8222) or 1-step Fix/Lyse Solution (Product #00-5333) with minimal impact on brightness and compensation. Clone-specific performance should be determined empirically.
Excitation: 488–561 nm
Emission: 607 nm
Laser: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD4 is a co-receptor for MHC class II molecules and a receptor for HIV. It is expressed mainly on T helper cells, as well as monocytes, macrophages, and dendritic cells. CD4 is found in thymus, lymph nodes, tonsils, spleen, and certain non-lymphoid tissues such as brain and gut. It plays a role in T-cell activation through association with the T-cell receptor complex and Lck kinase, and may mediate neuronal damage in immune-related CNS diseases. Multiple alternatively spliced transcripts have been identified (RefSeq, July 2017).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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