Thermo Fisher Scientific Phospho-EIF2S1 (Ser52) Polyclonal Antibody
EIF2S1 (Ser52) 인산화 형태를 검출하는 Rabbit Polyclonal Antibody로, Western blot에 적합함. 인간 및 랫트 반응성. 항원 친화 크로마토그래피로 정제됨. 단기 4°C, 장기 -20°C 보관 권장.
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Thermo Fisher Scientific Phospho-EIF2S1 (Ser52) Polyclonal Antibody
Applications
- Western Blot (WB): 1:500–1:3,000
Species Reactivity
- Human, Rat
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Carrier-protein conjugated synthetic peptide corresponding to residues around human eIF2 alpha (phospho Ser52) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.77 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 1% BSA, 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2792758 |
Product Specific Information
- Keep as concentrated solution.
- Positive Control:
- 293T
- 293T (25 µg/ml anisomycin treatment for 16 hr)
- HeLa
- HeLa (4 µg/ml Tunicamycin treatment for 16 hr)
- HepG2
- HepG2 (3 µM Thapsigargin treatment for 12 hr)
- PC-12 (300 µM Thapsigargin treatment for 8 hr)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
EIF2a (Eukaryotic translation initiation factor 2A) is a heterotrimer composed of three subunits (alpha, beta, and gamma). This translation initiation factor drives binding of initiator methionyl-tRNA to the 40S ribosome in an AUG-dependent manner to form the 43S pre-initiation complex. The polypeptide can be phosphorylated by related protein kinases activated in response to stress. Phosphorylated EIF2A inhibits EIF2B activity and prevents guanine nucleotide exchange.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(이미지 파일명: PA5-85618_EIF2S1_P05198-1_Rabbit.svg, PA5-85618_EIF2S1_P05198-1_Rabbit_PDP.jpeg)
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