Thermo Fisher Scientific Rat anti-Mouse IgG1 Secondary Antibody, Biotin, eBioscience
Biotin-conjugated rat monoclonal anti-mouse IgG1 secondary antibody. Flow cytometry, IHC, ICC 등 다양한 응용 가능. 높은 특이성과 비교차 반응성 보유. 0.5 mg/mL 액상 형태로 4°C 암소 보관.
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Thermo Fisher Scientific Rat anti-Mouse IgG1 Secondary Antibody, Biotin, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 10 µg/mL |
| Immunohistochemistry (Frozen) (IHC (F)) | 10 µg/mL |
| Immunocytochemistry (ICC/IF) | 10 µg/mL |
| Flow Cytometry (Flow) | Assay-Dependent |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rat / IgG |
| Class | Monoclonal |
| Type | Secondary Antibody |
| Clone | M1-14D12 |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11220482 |
| Target | IgG1 |
| Antibody Form | Whole Antibody |
| Filtration | 0.2 µm post-manufacturing filtered |
Product Specific Information
The monoclonal antibody M1-14D12 recognizes Mouse IgG1 antibodies and can be used as a second step reagent in flow cytometry and microscopy applications.
It does not recognize other mouse isotypes nor cross-react with rat IgG1 or any rat isotype antibodies.
Applications Reported:
This M1-14D12 antibody has been reported for use in:
- Flow cytometric analysis
- Immunohistochemical staining of frozen tissue sections
- Immunohistochemical staining of formalin-fixed, paraffin-embedded tissue sections
- Immunocytochemistry
Applications Tested:
Tested by immunocytochemistry on fixed and permeabilized cells using a mouse IgG1 primary antibody.
Recommended concentration ≤ 10 µg/mL; titrate for optimal assay performance.
Target Information
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins.
They are useful for detection, sorting, or purification of specific targets.
Secondary antibodies enhance sensitivity via signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
Typically generated by immunizing host animals with pooled mouse immunoglobulins and purified via immunoaffinity chromatography or other modifications to yield highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Additional Formats
Available conjugates include:
- APC
- eFluor 450, 570, 615, 660
- FITC
- HRP
- PE, PE-Cyanine7, PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436, 600, 780
- Custom conjugation available upon request
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