Thermo Fisher Scientific ATP6AP1 Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:1,000

Product Specifications

Product Specific Information

• Detects endogenous ATP6AP1 protein at a molecular weight of approximately 52 kDa.
• Purity >95% confirmed by SDS-PAGE.

Target Information

Vacuolar-type H⁺-ATPase (V-ATPase) is a multisubunit enzyme responsible for acidifying eukaryotic intracellular organelles. It pumps protons against an electrochemical gradient to synthesize ATP.
V-ATPase consists of:

• V1 domain: responsible for ATP hydrolysis (subunits A–H)
• V0 domain: responsible for proton translocation (subunits a, d, c, c′, c″)

ATP6AP1 (ATPase, H⁺ transporting, lysosomal accessory protein 1) — also known as 16A, CF2, Ac45, XAP3, ATP6S1, VATPS1, or ATP6IP1 — is a type I transmembrane accessory protein of V-ATPase, predominantly expressed in endocrine and neuronal cells.
ATP6AP1 targets the V-ATPase enzyme to specialized vacuolar systems and interacts with V0 domain subunits via its cytoplasmic tail. Disruption of this interaction in osteoclasts results in impaired bone resorption, indicating its crucial role in osteoclastic function.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.