Thermo Fisher Scientific Phospho-FAK (Tyr576) Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• Positive Control: Neuro2A, C8D30, NIH-3T3, C2C12, Rat2, HeLa, 293T, 293T treated with 50 ng/mL EGF, HepG2, HepG2 treated with 200 ng/mL EGF
• Predicted Reactivity: Xenopus laevis (100%), Chicken (100%)
• Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Focal Adhesion Kinase (FAK) is a 125 kDa non-receptor protein tyrosine kinase that acts as a substrate for Src and is a key element of integrin signaling. FAK plays an important role in cell spreading, differentiation, migration, cell death, and acceleration of the G1 to S phase transition of the cell cycle. It contains a central catalytic domain and a C-terminal tail that localizes it to focal adhesions, sites where cells attach to the extracellular matrix via surface integrin receptors.

Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin. Adhesion of murine NIH3T3 fibroblasts to fibronectin promotes association of the Grb2 adapter protein and c-Src PTK with FAK in vivo, resulting in activation of the ERK2 MAP kinase. In v-Src-transformed NIH3T3 cells, the association of v-Src, Grb2, and Sos with FAK is independent of cell adhesion to fibronectin. In vitro, the Grb2 SH2 domain binds directly to tyrosine-phosphorylated FAK, with Tyr925 identified as the binding site by site-directed mutagenesis. Several transcript variants encoding different isoforms have been identified for the FAK gene.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.