Thermo Fisher Scientific Paxillin Polyclonal Antibody
Rabbit polyclonal antibody recognizing human Paxillin. Validated for Western blot at 1:500–1:3,000 dilution. Affinity purified and supplied in PBS with BSA and glycerol. Suitable for research use in cell adhesion and signaling studies.
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Applications
- Western Blot (WB): 1:500–1:3,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Paxillin. The exact sequence is proprietary. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 1% BSA, 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2736327 |
Product Specific Information
- Positive Control: K562, A431, HeLa, HepG2
- Store product as a concentrated solution.
- Centrifuge briefly prior to opening the vial.
Target Information
Paxillin is a focal adhesion protein and a substrate for several tyrosine kinases such as src, FAK, and p210BRC/ABL. The tyrosine phosphorylation of paxillin is affected by conditions that change cell-cell adhesion. Paxillin associates tightly with FAK and Crk through its SH2 domain, independent of the extracellular matrix. Although initially discovered in fibroblasts, its phosphorylation may also play a role during neurite extension and differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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