Thermo Fisher Scientific Goat anti-Canine IgG (H+L) Secondary Antibody, AP
염색 및 단백질 검출용 염소 유래 항-개 IgG(AP) 2차 항체. ELISA, Western blot, IHC 등 다양한 응용에 적합. 높은 특이성과 감도를 위해 친화 크로마토그래피로 정제됨. 장기 보관 시 -20°C에서 안정적 유지.
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Thermo Fisher Scientific Goat anti-Canine IgG (H+L) Secondary Antibody, AP
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:20,000 |
| Immunohistochemistry (IHC) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Dog |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | AP |
| Form | Liquid |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 30 mM triethanolamine, 1% BSA, 5 mM MgCl₂, 0.1 mM ZnCl₂ |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2535543 |
| Target | IgG |
| Antibody Form | Whole Antibody |
Additional Formats Available
- Biotin conjugate
- FITC conjugate
- HRP conjugate
- Custom conjugation available upon request
Product Specific Information
Each lot’s sensitivity is confirmed using ELISA, and specificity is validated by immunoelectrophoresis (IEP).
Target Information
Anti-Canine secondary antibodies are affinity-purified with well-characterized specificity for canine immunoglobulins. They are useful in detection, sorting, or purification of target proteins.
Secondary antibodies enhance versatility and sensitivity by enabling multiple detection systems (HRP, AP, fluorescence) and signal amplification.
Typically, secondary antibodies are produced by immunizing the host animal with pooled immunoglobulins from the target species, followed by purification and modification (e.g., immunoaffinity chromatography, fragmentation, conjugation) to yield highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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