Thermo Fisher Scientific Goat anti-Canine IgG (H+L) Secondary Antibody, FITC
개 IgG(H+L)에 특이적인 염소 유래 폴리클로날 2차 항체로, FITC 형광 표지. Western blot, ICC/IF, 유세포분석 등 다양한 응용에 적합. 높은 특이성과 감도로 연구용에 최적화됨. 4°C 보관, 장기 보관 시 -20°C 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,500 |
| Immunocytochemistry (ICC/IF) | 1:500–1:20,000 |
| Flow Cytometry (Flow) | 1:500–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobulins Heavy and Light chains |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.8, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2535541 |
| Target | IgG |
| Antibody Form | Whole Antibody |
Additional Formats Available
- AP
- Biotin
- HRP
- Custom conjugation available upon request
Product Specific Information
The sensitivity of each lot of antibody is confirmed using ELISA.
The specificity of each lot of antibody is confirmed by immunoelectrophoresis (IEP).
Target Information
Anti-Canine secondary antibodies are affinity-purified antibodies with well-characterized specificity for canine (dog) immunoglobulins.
They are useful for detection, sorting, or purification of the specified target.
Secondary antibodies provide versatility and higher sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
These antibodies are typically produced by immunizing the host animal with pooled canine immunoglobulins and further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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