Thermo Fisher Scientific Phospho-c-Myc (Thr58) Polyclonal Antibody
Human, Mouse, Rat 반응성을 가진 Rabbit Polyclonal 항체로, Thr58 부위의 c-Myc 인산화 형태를 검출. Western blot, IHC, ELISA에 사용 가능. 고순도 Affinity chromatography 정제, PBS/glycerol buffer에 보존.
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Thermo Fisher Scientific Phospho-c-Myc (Thr58) Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:100–1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic phosphorylated peptide around T58 of mouse MYC (NP_0011708231) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.49 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2901807 |
Product Specific Information
- Positive Samples: 293T
- Immunogen sequence: LPTPP
Target Information
The c-Myc protein is a 62 kDa transcription factor encoded by the c-Myc gene on human chromosome 8q24. It is commonly activated in various tumor cells and plays a critical role in cellular proliferation, differentiation, apoptosis, and cell cycle progression.
Phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin-dependent kinase, ERK2, and JNK regulates cell proliferation and cycle.
c-Myc is essential for vasculogenesis and angiogenesis during tumor development. The deregulated expression of c-Myc induces apoptosis and contributes to neoplastic transformation. Amplification or translocation of the c-Myc gene is associated with multiple human cancers including lung, breast, colon, and Burkitt's lymphoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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