Thermo Fisher Scientific Glu-Glu Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:20,000

-

Immunocytochemistry (ICC/IF)

1:100-1:400

-

ELISA (ELISA)

1:1,000-1:30,000

-

Immunoprecipitation (IP)

1-4 µg/mg

-

Product Specifications

Species Reactivity

Tag

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to aa 314-319 (EYMPME) of middle T antigen of mouse polyomavirus conjugated to KLH.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

2-8°C

Shipping conditions

Ambient (domestic); Wet ice (international)

Target Information

The Glu-Glu tag, also known as the EYMPME sequence, is a short peptide epitope tag consisting of two glutamic acid (Glu) residues interspersed with various amino acids. It is typically incorporated into the N-terminus of proteins through subcloning into vectors such as the pFASTBAC vector. This tag is particularly useful in recombinant protein expression and purification processes. It facilitates the detection and isolation of tagged proteins via specific antibodies that recognize the Glu-Glu sequence. The Glu-Glu tag can be utilized in various systems, including baculovirus expression in insect cells, and provides efficient means for studying protein-protein interactions, localization, and function.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.