Thermo Fisher Scientific Glu-Glu Polyclonal Antibody
Glu-Glu 태그 단백질 검출용 토끼 폴리클로날 항체. Western blot, ICC/IF, ELISA, IP 등 다양한 응용에 적합. 합성 펩타이드 EYMPME 면역원 사용. 고순도 Protein A 정제, 액상 형태로 2–8°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:20,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:400 |
| ELISA | 1:1,000–1:30,000 |
| Immunoprecipitation (IP) | 1–4 µg/mg |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Tag |
| Host/Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to aa 314–319 (EYMPME) of middle T antigen of mouse polyomavirus conjugated to KLH |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.09% sodium azide |
| Storage Conditions | 2–8°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The Glu-Glu tag (EYMPME) is a short peptide epitope tag consisting of two glutamic acid (Glu) residues interspersed with other amino acids. It is typically incorporated into the N-terminus of recombinant proteins through subcloning into expression vectors such as the pFASTBAC vector. This tag enables efficient detection and purification of tagged proteins via specific anti–Glu-Glu antibodies. It is widely used in recombinant protein expression systems, including baculovirus-insect cell systems, to study protein localization, interactions, and function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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