Thermo Fisher Scientific Glu-Glu Polyclonal Antibody
Thermo Fisher Scientific의 Glu-Glu Polyclonal Antibody는 EYMPME 서열을 인식하는 항체로, 단백질 발현 및 정제 과정에서 Glu-Glu 태그 검출에 적합합니다. WB, ICC/IF, ELISA, IP 등 다양한 응용에 사용 가능하며, Goat 유래의 Polyclonal 형태로 높은 특이성과 재현성을 제공합니다.
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Thermo Fisher Scientific Glu-Glu Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:20,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:400 |
| ELISA | 1:1,000–1:30,000 |
| Immunoprecipitation (IP) | 1–4 µg/mg |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host/Isotype | Goat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to aa 314–319 (EYMPME) of middle T antigen of mouse polyomavirus conjugated to KLH |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.09% sodium azide |
| Storage Conditions | –20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The Glu-Glu tag, also known as the EYMPME sequence, is a short peptide epitope tag consisting of two glutamic acid (Glu) residues interspersed with various amino acids. It is typically incorporated into the N-terminus of proteins through subcloning into vectors such as the pFASTBAC vector.
This tag is particularly useful in recombinant protein expression and purification processes. It facilitates the detection and isolation of tagged proteins via specific antibodies that recognize the Glu-Glu sequence.
The Glu-Glu tag can be utilized in various systems, including baculovirus expression in insect cells, and provides efficient means for studying protein-protein interactions, localization, and function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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