Thermo Fisher Scientific Topo II Alpha Polyclonal Antibody
Rabbit polyclonal antibody recognizing human Topo II alpha for IHC and IHC-P applications. Antigen affinity purified with 0.25 mg/mL concentration. Suitable for detecting DNA synthesis and mitosis markers. For research use only.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (IHC) | 1:100–1:500 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:100–1:500 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 1500 and the C-terminus (residue 1531) of Human Topoisomerase (DNA) II Alpha |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.25 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | TBS, pH 7.0–8.0, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Based on 100% sequence identity, predicted to react with Orangutan, Monkey, Gorilla, Chimpanzee, Northern white-cheeked gibbon, and Crab-eating macaque
Target Information
Topo II alpha plays key roles in DNA synthesis, transcription, and chromosomal segregation during mitosis.
It serves as a sensitive and specific marker of late S-, G2-, and M-phases in transformed and developmentally regulated normal cells.
Topo II alpha is also implicated in drug resistance of tumor cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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