Thermo Fisher Scientific Ki-67 Monoclonal Antibody (MKI67, 2462)

Applications and Tested Dilution

Product Specifications

Product Specific Information

• For Immunohistochemistry (PFA fixed): incubate antibody for 30 min at room temperature.
• Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C, followed by cooling at room temperature for 20 minutes.

Target Information

Ki-67 is a nuclear protein expressed during various stages of the cell cycle, particularly during late G1, S, G2, and M phases. It contains a forkhead-associated (FHA) domain that mediates association with euchromatin at the perichromosomal layer, centromeric heterochromatin, and nucleolus.

• Ki-67 shows cell cycle-dependent localization:
  • Perinucleolar expression at G1
  • Nuclear matrix expression at G2
  • Chromosomal expression during M phase
• Commonly used as a proliferation marker since it is absent in G0 cells and increases from G1 through mitosis.
• Useful for determining the cell growth fraction in neoplasms.
• Correlates with tumor grade; low Ki-67 index indicates low-grade tumors.
• Routinely used as a neuronal marker of cell cycling and proliferation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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