
Thermo Fisher Scientific Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, HRP
Western blot, IHC, ELISA 등 다양한 응용에 적합한 HRP 결합형 염소 유래 항-토끼 IgG 보조항체. 높은 교차흡착도로 비특이적 반응 최소화. 동결건조 형태로 안정적 보관 가능하며, 친화도 정제되어 높은 순도 제공.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:20,000 |
| Immunohistochemistry (IHC) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rabbit |
| Host/Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Purified Rabbit IgG, whole molecule |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.1% ProClin 150 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2534776 |
| Target | IgG |
| Cross Adsorption | Against bovine, human and mouse IgG |
| Antibody Form | Whole Antibody |
Product Specific Information
- Rehydrate with 1.1 mL of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.)
- Centrifuge to remove particulates and prepare fresh working dilution daily.
- Store lyophilized material at 2–8°C. For long-term storage after reconstitution, dilute with 50% glycerol and store at -20°C as a liquid.
- Based on immunoelectrophoresis, no reactivity is observed to non-immunoglobulin rabbit serum proteins, or serum proteins from bovine, human, or mouse.
- Reactivity confirmed with heavy (gamma) chains on rabbit IgG and light chains on all rabbit immunoglobulins.
- Affinity purified antibody is >95% pure based on SDS-PAGE.
Target Information
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins. They are useful for detection, sorting, or purification of rabbit IgG targets. Secondary antibodies enhance assay versatility and sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody. These reagents are commonly produced by immunizing the host animal with pooled immunoglobulins from the target species and further purified or conjugated for specific applications.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Available Conjugate Formats
다양한 형광 및 효소 표지 형식으로 제공됩니다:
- Alexa Fluor 350, 405, 430, 488, 514, 532, 546, 555, 568, 594, 633, 635, 647, 660, 680, 700, 750, 790
- AP, APC, Biotin, Biotin-XX
- Cyanine3, Cyanine5
- DyLight 350, 405, 488, 550, 594, 633, 650, 680, 755, 800
- FITC, Pacific Blue, Pacific Orange
- PE, PE-Alexa Fluor 610, PE-Alexa Fluor 647
- Rhodamine, Rhodamine Red-X, Texas Red, Texas Red-X, TRITC
Custom conjugation available upon request.
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