Thermo Fisher Scientific Phospho-Tie2 (Ser1119) Polyclonal Antibody
인간, 마우스, 랫트 반응성을 가지는 Rabbit Polyclonal 항체로 Ser1119 위치의 인산화 Tie2 단백질을 검출. WB, IHC, ICC, ELISA 등 다양한 응용에 적합. Protein A 정제, 1 mg/mL 농도, -20°C 보관.
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Thermo Fisher Scientific Phospho-Tie2 (Ser1119) Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic phosphopeptide derived from human Tie2 around the phosphorylation site of Ser1119 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The TEK receptor tyrosine kinase (also known as TIE2) is expressed almost exclusively in endothelial cells. TEK signaling is crucial for endothelial cell–smooth muscle cell communication in venous morphogenesis, and defects in TEK are associated with inherited venous malformations. The ligand angiopoietin-1 binds to this receptor and mediates a signaling pathway involved in embryonic vascular development. Immunoblotting studies have shown that TIE2 expression increases in response to thyroid-stimulating hormone and agents that elevate intracellular cAMP. Hematopoietic stem cells (HSCs) expressing TIE2 are quiescent, antiapoptotic, and form a side population that adheres to osteoblasts within the bone marrow niche.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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