Thermo Fisher Scientific NLRP3 Polyclonal Antibody
NLRP3 단백질을 인식하는 Thermo Fisher Scientific의 rabbit polyclonal antibody로, WB, IHC, ICC 등 다양한 응용에 사용 가능. 고순도 항원 친화 크로마토그래피로 정제되었으며, 인간 및 설치류 반응성. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.5 µg/mL | View 11 publications |
| Immunohistochemistry (IHC) | – | View 8 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–5 µg/mL | View 2 publications |
| Immunocytochemistry (ICC/IF) | – | View 4 publications |
| Flow Cytometry (Flow) | 1–3 µg/1×10⁶ cells | View publications |
| Immunoprecipitation (IP) | – | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Rat |
| Published Species | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to human CIAS1 (N-terminus, 12–31aa: RYLEDLEDVDLKKFKMHLED) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 500 µg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 4 mg trehalose |
| Contains | No preservative |
| Storage Conditions | –20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2746855 |
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
This gene encodes a pyrin-like protein containing a pyrin domain, a nucleotide-binding site domain, and a leucine-rich repeat motif. The protein interacts with apoptosis-associated speck-like protein PYCARD/ASC and is part of the NALP3 inflammasome complex, which activates NF-kappaB signaling. It regulates inflammation, immune response, and apoptosis. Mutations are linked to familial cold autoinflammatory syndrome and neonatal-onset multisystem inflammatory disease. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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