Thermo Fisher Scientific CD11b Monoclonal Antibody (M1/70), Alexa Fluor 700, eBioscience
Mouse CD11b (M1/70) 단클론 항체로, Alexa Fluor 700으로 표지되어 있으며 흐름세포분석(Flow Cytometry)에 최적화되어 있습니다. Mac-1 복합체 인식, 다양한 면역세포 표면 CD11b 검출에 사용됩니다. 0.25 µg/test 권장, 4°C 암소 보관.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 44 publications |
| Immunohistochemistry (PFA fixed) | - | View 4 publications |
| Immunohistochemistry (Frozen) | - | View 2 publications |
| Immunocytochemistry (ICC/IF) | - | View 17 publications |
| Flow Cytometry (Flow) | 0.25 µg/test | View 286 publications |
| Immunoprecipitation (IP) | - | View 1 publication |
| Neutralization (Neu) | - | View 2 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Fish, Human, Mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), Alexa Fluor™ 700, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | M1/70 |
| Conjugate | Alexa Fluor™ 700 |
| Excitation / Emission Max | 696 / 719 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_657586 |
Available Formats
- Unconjugated
- Alexa Fluor 488, 532, 660
- APC, APC-eFluor 780
- Biotin, Brilliant UV Series (395–805)
- Brilliant Violet Series (421–786)
- eFluor 450, 506, 660
- FITC, PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright Series (436–780)
- NovaFluor Blue / Yellow Series
- Functional Grade
- Request custom conjugation
Product Specific Information
Description:
The M1/70 monoclonal antibody reacts with mouse CD11b, a 165–170 kDa integrin alphaM. CD11b non-covalently associates with CD18 to form the alphaMbeta2 integrin (Mac-1) and binds CD54 (ICAM-1), C3bi, and fibrinogen. Mac-1 is expressed by macrophages, NK cells, granulocytes, activated lymphocytes, and B-1 cells in the peritoneal cavity. M1/70 also cross-reacts with human CD11b and can detect this antigen on human peripheral blood monocytes, granulocytes, and some NK cells. CD11b participates in adhesive cell interactions.
Applications Reported:
Reported for use in flow cytometric analysis.
Applications Tested:
Tested by flow cytometric analysis of mouse splenocyte or bone marrow cell suspensions. Recommended ≤0.25 µg per test (in 100 µL final volume). Cell number range: 10⁵–10⁸ cells/test. Optimize titration for best performance.
Fluorochrome Information:
Alexa Fluor® 700 emits at 723 nm; excitation with red laser (633 nm). Most instruments require a 685 LP mirror and 710/20 filter.
Excitation: 633–647 nm; Emission: 723 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD11b (integrin alpha-M, ITGAM):
A 165 kDa adhesion molecule that associates with integrin beta-2 (CD18) to form the CD11b/CD18 complex (Mac-1, CR3). Expressed on monocytes/macrophages, granulocytes, activated lymphocytes, subsets of NK and dendritic cells, and microglia. Functions as a receptor for ICAM-1, ICAM-2, ICAM-4, CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X, mediating cell-cell and cell-matrix interactions. Expression varies by cell type and tissue; used as a microglial marker in nervous tissue. CD11b dysfunction is linked to systemic lupus erythematosus and ITGAM-related susceptibility.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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