Thermo Fisher Scientific CD8a Monoclonal Antibody (RPA-T8), Brilliant Violet 605, eBioscience

Thermo Fisher Scientific CD8a Monoclonal Antibody (RPA-T8), Brilliant Violet 605

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.25 µg)/test

Product Specifications

Additional Formats Available

• Alexa Fluor 532
• Alexa Fluor 561
• Alexa Fluor 660
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Brilliant UV 395 / 563 / 615 / 661 / 737 / 805
• Brilliant Violet 421 / 480 / 650 / 711 / 786
• eFluor 450 / 506
• FITC
• NovaFluor Blue 610-70S / 660-120S
• NovaFluor Red 710
• NovaFluor Yellow 610 / 660
• PE / PE-Cyanine5 / PE-Cyanine5.5 / PE-Cyanine7 / PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436 / 600 / 645 / 702 / 780
• Request custom conjugation available

Product Specific Information

The RPA-T8 monoclonal antibody reacts with human CD8a, a 32–34 kDa cell surface receptor expressed as CD8 alpha/beta heterodimer or CD8 alpha/alpha homodimer. CD8a is found on most thymocytes, mature T cells, and NK cells. It binds MHC class I molecules and associates with protein tyrosine kinase p56lck, contributing to T-cell development and activation.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-diluted and tested on normal human peripheral blood cells at 5 µL (0.25 µg)/test
• Recommended Use: Final volume 100 µL; 10⁵–10⁸ cells/test

Brilliant Violet™ 605 (BV605) emits at 605 nm and is used with a violet (405 nm) laser. Ensure instrument compatibility.

Buffer Recommendations:
When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific interactions.

Light Sensitivity:
BV605 is sensitive to photo-induced oxidation. Protect vial and samples from light.

Fixation Compatibility:
Samples may be stored in IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days at 4°C in the dark. BV605 is not compatible with methanol-based fixation.

Excitation: 407 nm
Emission: 605 nm
Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

Cluster of differentiation 8 (CD8) is a type I transmembrane glycoprotein of the immunoglobulin receptor family, crucial for T-cell signal transduction, differentiation, and activation. CD8 acts as a co-receptor for MHC class I molecules, while CD4 binds MHC class II. CD8 is expressed on T cells, NK cells, monocytes, and dendritic cells, forming disulfide-linked dimers of CD8α and CD8β or CD8αα. MHC-I/peptide complex binding recruits Lck kinase, leading to lymphokine production and cytotoxic T lymphocyte (CTL) activation. Activated CTLs contribute to pathogen and tumor clearance. Differentiation of naive CD8+ T cells into CTLs is enhanced by IL-2, IL-12, and TGF-β1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.