Thermo Fisher Scientific CD8a Monoclonal Antibody (RPA-T8), Brilliant Violet 605, eBioscience
인간 CD8a 항원을 인식하는 RPA-T8 클론의 단클론 항체로, Brilliant Violet 605 형광체가 결합된 제품입니다. Flow Cytometry용으로 최적화되어 있으며, 405 nm 레이저에서 605 nm 방출. 광산화에 민감하므로 암조건 보관 필요.
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Thermo Fisher Scientific CD8a Monoclonal Antibody (RPA-T8), Brilliant Violet 605
Applications
- Flow Cytometry (Flow)
Tested Dilution: 5 µL (0.25 µg)/test
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | RPA-T8 |
| Conjugate | Brilliant Violet™ 605 |
| Excitation/Emission Max | 406 / 603 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2937152 |
Additional Formats Available
- Alexa Fluor 532
- Alexa Fluor 561
- Alexa Fluor 660
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Brilliant UV 395 / 563 / 615 / 661 / 737 / 805
- Brilliant Violet 421 / 480 / 650 / 711 / 786
- eFluor 450 / 506
- FITC
- NovaFluor Blue 610-70S / 660-120S
- NovaFluor Red 710
- NovaFluor Yellow 610 / 660
- PE / PE-Cyanine5 / PE-Cyanine5.5 / PE-Cyanine7 / PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright 436 / 600 / 645 / 702 / 780
- Request custom conjugation available
Product Specific Information
The RPA-T8 monoclonal antibody reacts with human CD8a, a 32–34 kDa cell surface receptor expressed as CD8 alpha/beta heterodimer or CD8 alpha/alpha homodimer. CD8a is found on most thymocytes, mature T cells, and NK cells. It binds MHC class I molecules and associates with protein tyrosine kinase p56lck, contributing to T-cell development and activation.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Pre-diluted and tested on normal human peripheral blood cells at 5 µL (0.25 µg)/test
- Recommended Use: Final volume 100 µL; 10⁵–10⁸ cells/test
Brilliant Violet™ 605 (BV605) emits at 605 nm and is used with a violet (405 nm) laser. Ensure instrument compatibility.
Buffer Recommendations:
When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific interactions.
Light Sensitivity:
BV605 is sensitive to photo-induced oxidation. Protect vial and samples from light.
Fixation Compatibility:
Samples may be stored in IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days at 4°C in the dark. BV605 is not compatible with methanol-based fixation.
Excitation: 407 nm
Emission: 605 nm
Laser: Violet Laser
BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.
Target Information
Cluster of differentiation 8 (CD8) is a type I transmembrane glycoprotein of the immunoglobulin receptor family, crucial for T-cell signal transduction, differentiation, and activation. CD8 acts as a co-receptor for MHC class I molecules, while CD4 binds MHC class II. CD8 is expressed on T cells, NK cells, monocytes, and dendritic cells, forming disulfide-linked dimers of CD8α and CD8β or CD8αα. MHC-I/peptide complex binding recruits Lck kinase, leading to lymphokine production and cytotoxic T lymphocyte (CTL) activation. Activated CTLs contribute to pathogen and tumor clearance. Differentiation of naive CD8+ T cells into CTLs is enhanced by IL-2, IL-12, and TGF-β1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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