Thermo Fisher Scientific CD11b Monoclonal Antibody (M1/70), Brilliant Violet 605, eBioscience

Thermo Fisher Scientific CD11b Monoclonal Antibody (M1/70), Brilliant Violet 605, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.25 µg/test
Publications: - References

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 488, 532, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395–805
• Brilliant Violet 421–786
• eFluor 450, 506, 660
• FITC
• Functional Grade
• NovaFluor Blue/Yellow series
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436–780
• Request custom conjugation available

Product Specific Information

The M1/70 monoclonal antibody reacts with mouse CD11b (integrin alphaM, 165–170 kDa). CD11b associates with CD18 to form the alphaMbeta2 integrin (Mac-1), binding CD54 (ICAM-1), C3bi, and fibrinogen. It is expressed by macrophages, NK cells, granulocytes, activated lymphocytes, and mouse B-1 cells.
M1/70 also cross-reacts with human CD11b, useful for detecting this antigen on human monocytes, granulocytes, and NK cell subsets.

Applications Reported: Flow cytometric analysis
Applications Tested: Mouse bone marrow cells (≤0.25 µg/test). Optimal titration recommended.

Brilliant Violet™ 605 (BV605):

• Tandem dye emitting at 605 nm, for use with 405 nm violet laser.
• Sensitive to light; protect samples and vials from light.
• Not compatible with methanol-based fixation.
• Compatible with fixation using IC Fixation Buffer or 1-step Fix/Lyse Solution for up to 3 days at 4°C in dark.

Buffer Recommendations:
Use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) when using multiple polymer dyes to minimize non-specific interactions.

Excitation / Emission / Laser:
407 nm / 605 nm / Violet Laser

Trademark Notice:
BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

CD11b (integrin alpha-M, ITGAM) forms a heterodimer with CD18 (integrin beta-2), known as Mac-1 or CR3. Expressed on monocytes/macrophages, granulocytes, lymphocytes, NK cells, dendritic cells, and microglia.
Functions as a receptor for ICAM-1, ICAM-2, ICAM-4, CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X — mediating critical cell-cell and cell-matrix interactions.
Used as a microglial marker in nervous tissue; expression varies with macrophage type and tissue origin.
Associated with systemic lupus erythematosus and ITGAM-related susceptibility.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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