Thermo Fisher Scientific CD11b Monoclonal Antibody (M1/70), Brilliant Violet 605, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.25 µg/test

Product Specifications

Available Conjugate Formats

• Unconjugated
• Alexa Fluor 488, 532, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395–805 series
• Brilliant Violet 421–786 series
• eFluor 450, 506, 660
• FITC
• Functional Grade
• NovaFluor Blue / Yellow series
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436–780 series
• Custom conjugation available upon request

Product Specific Information

The M1/70 monoclonal antibody reacts with mouse CD11b (integrin alphaM, 165–170 kDa). CD11b associates with CD18 to form the alphaMbeta2 integrin (Mac-1), binding to CD54 (ICAM-1), C3bi, and fibrinogen. It is expressed on macrophages, NK cells, granulocytes, activated lymphocytes, and B-1 cells in the peritoneal cavity. M1/70 also cross-reacts with human CD11b, enabling detection on human monocytes, granulocytes, and NK cell subsets.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Mouse bone marrow cells at ≤0.25 µg/test (100 µL final volume)
• Recommended: Optimize titration for best performance

Fluorochrome Information:
Brilliant Violet™ 605 (BV605) emits at 605 nm, excitable by a 405 nm laser. Ensure instrument compatibility.

Staining Recommendations:
When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific interactions.

Light Sensitivity:
Protect from light due to photo-induced oxidation sensitivity.

Fixation Compatibility:
Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days at 4°C in the dark. BV605 is not compatible with methanol-based fixation.

Optical Properties:
Excitation: 407 nm
Emission: 605 nm
Laser: Violet

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

CD11b (integrin alpha-M, ITGAM) is a 165 kDa adhesion molecule forming a heterodimer with CD18 (integrin beta-2). The CD11b/CD18 complex (Mac-1, CR3) mediates adhesion via binding to ICAM-1, ICAM-2, ICAM-4, CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X.
CD11b is expressed on monocytes/macrophages, granulocytes, activated lymphocytes, NK cells, dendritic cells, and microglia. Expression increases with monocyte maturation and varies among macrophage subsets.
CD11b dysfunction is associated with systemic lupus erythematosus and ITGAM-related susceptibility to autoimmune disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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