Thermo Fisher Scientific TRIM28 Polyclonal Antibody
TRIM28 단백질을 인식하는 Rabbit Polyclonal Antibody로, Western blot, IHC, ICC, ChIP 등 다양한 응용에 적합합니다. Human, Mouse, Rat에 반응하며 항원 친화 크로마토그래피로 정제되었습니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:10,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
| ChIP assay (ChIP) | 2.5 µg/10^6 cells |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 36–226 of Human KAP1 (TRIM28, UniProt ID: Q13263-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.31 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.1M Tris glycine, pH 7, with 10% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2545125 |
Product Specific Information
- Recommended positive controls: A431, H1299, HeLa, HepG2, NIH-3T3, mouse brain
- Predicted reactivity: Mouse (91%), Rat (91%), Bovine (94%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The KRAB (Kruppel-associated box) domain is about 45 amino acids in length and functions as a transcriptional repression domain found in numerous transcription factors. Over 220 KRAB-zinc finger protein (KRAB-ZFP) genes have been identified in the human genome. These proteins repress transcription via interactions with KAP-1 (KRAB-associated protein 1).
KAP-1 is an 835 amino acid polypeptide containing a RING finger, B boxes, and a PHD finger. It forms complexes with KRAB-domain transcription factors to enhance repression efficiency and interacts directly with HP1 (heterochromatin protein 1) and KRAZ1 (Kruppel-associated box-containing zinc finger protein 1).
KAP-1 targets KRAZ1 to centromeric heterochromatin containing HP1α, regulating transcriptional repression. Mutant KAP-1 unable to bind HP1 results in random KRAZ1 distribution and strong transcriptional activation.
Safety Information
⚠ WARNING: This product can expose you to chemicals including mercury, known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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