Thermo Fisher Scientific TRIM28 Polyclonal Antibody
TRIM28 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, IHC, ICC, IP, ChIP 등 다양한 응용에 사용 가능. Human, Mouse, Rat 반응성. 항원 친화 크로마토그래피로 정제되었으며, 0.48 mg/mL 농도의 액상 형태로 제공. 연구용 전용 제품.
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- PA527648
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000–1:10,000 | View 2 publications |
| Immunohistochemistry (IHC) | – | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | – |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | – |
| Flow Cytometry (Flow) | – | View 2 publications |
| Immunoprecipitation (IP) | 1:500–1:1,000 | – |
| ChIP assay (ChIP) | 2.5 µg/10^6 cells | – |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 361–613 of Human KAP1 (TRIM28) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.48 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol, 1% BSA |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2545124 |
Product Specific Information
- Recommended positive controls: H1299, HeLa, NIH-3T3
- Predicted reactivity: Mouse (91%), Rat (91%), Bovine (93%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The KRAB (Kruppel-associated box) domain is about 45 amino acids in length and functions as a transcriptional repression domain found in numerous transcription factors. Over 220 KRAB-zinc finger protein (KRAB-ZFP) genes have been identified in the human genome. These proteins repress transcription via interactions with KAP-1 (KRAB-associated protein 1).
KAP-1 is an 835 amino acid polypeptide containing a RING finger, B boxes, and a PHD finger. It forms complexes with KRAB-domain transcription factors to enhance repression efficiency. KAP-1 interacts directly with HP1 (heterochromatin protein 1) and KRAZ1 (Kruppel-associated box-containing zinc finger protein 1), targeting KRAZ1 to centromeric heterochromatin regions containing HP1α, thereby contributing to transcriptional regulation. Mutant KAP-1 proteins that bind KRAB but not HP1 cause random KRAZ1 distribution and strong transcriptional activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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