Thermo Fisher Scientific UBE2B Recombinant Rabbit Monoclonal Antibody (JE56-12)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Product Specifications

Species Reactivity

Human, Zebrafish

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

JE56-12

Immunogen

Synthetic peptide within N-terminal Human Ube2B if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:UBE2B,uniProtId:P63146-1,ncbiNodeId:9606,antigenRange:1,antigenLength:152,antigenImageFileName:MA5-42490_UBE2B_P63146-1_Rabbit.svg,antigenImageFileNamePDP:MA5-42490_UBE2B_P63146-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 40% glycerol

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2911631

Product Specific Information

Positive Control: A431 cell lysate, THP-1 cell lysate, HepG2 cell lysate, zebrafish tissue lysates, human breast carcinoma tissue, human fetal skeletal muscle tissue.

Subcellular Location: Nucleus, Cell membrane.

Target Information

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2B is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Its protein sequence is 100% identical to the mouse, rat, and rabbit homologs, which indicates that this enzyme is highly conserved in eukaryotic evolution.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.