Thermo Fisher Scientific TCP1 Polyclonal Antibody
TCP1 단백질을 인식하는 Thermo Fisher Scientific의 염소 유래 다클론 항체로, Western blot, IHC, ICC/IF, Flow Cytometry에 적합. 합성 펩타이드 항원을 기반으로 제작되었으며, 0.5 mg/mL 농도의 액상 형태로 제공. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.3–1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 10 µg/mL |
| Flow Cytometry (Flow) | 10 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide sequence (SYEDAVHSGALND) corresponding to the C-terminus amino acids of TCP1 (aa 544–556) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Ammonium sulfate precipitation |
| Storage Buffer | TBS, pH 7.3, with 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | –20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_10985037 |
Product Specific Information
This antibody is tested in Peptide ELISA: antibody detection limit dilution 32,000.
Target Information
The T Complex Polypeptide 1 (TCP-1) is an approximately 60 kDa protein constitutively expressed in almost all eukaryotic cells and is upregulated during spermatogenesis. It is found in the cytosol as a subunit of a hetero-oligomeric chaperone involved in the folding of actin and tubulin.
Chaperonins recognize and stabilize polypeptide intermediates during folding, assembly, and disassembly. They share similarities with Heat Shock Protein 70 (HSP70), including high abundance, induction by environmental stress, and ATPase activity.
The chaperonin family includes mitochondrial HSP60, E. coli GroEL, the plastid Rubisco-subunit binding protein, and the archaebacterial protein TF55. The TCP-1 sequence shows nearly 40% identity to TF55 but only minimal similarity to HSP60 and GroEL.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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