Thermo Fisher Scientific IL-1 beta Monoclonal Antibody (A7F9)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunocytochemistry (ICC/IF)

1:100

-

ELISA (ELISA)

1:20,000

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

A7F9

Immunogen

Recombinant protein within Human IL-1 beta aa 117-269/269. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:IL-1 beta,uniProtId:P01584-1,ncbiNodeId:9606,antigenRange:117-269,antigenLength:269,antigenImageFileName:MA5-50556_IL-1_beta_P01584-1_House_mouse.svg,antigenImageFileNamePDP:MA5-50556_IL-1_beta_P01584-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 0.1% BSA, 40% glycerol

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_3091034

Product Specific Information

Positive control: THP-1 treated with 80 nm TPA overnight then treated with 100 ng/mL LPS for 6 hours and 300 ng/mL BFA for 3 hours whole cell lysate.

Predicted band size: 31 kDa

Subcellular Location: Extracellular exosome, Secreted, Lysosome, Cytosol.

Product MA5-50556 was used in an Indirect ELISA.

Target Information

Interleukin-1 beta (IL-1 beta) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 beta is synthesized in response to inflammatory stimuli as a 31 kDa inactive pro-form that accumulates in the cytosol. Cleavage of pro-IL-1 beta into the active 17 kDa protein requires the activation of inflammasomes, which are multi-protein complexes that respond to pathogens, stress conditions, and other danger signals. Inflammasome activation triggers the processing of the caspase-1 precursor into its active form, which in turn cleaves pro-IL-1 beta. IL-1 beta lacks a signal sequence peptide for classical ER/Golgi pathway and is secreted alongside caspase-1 via an alternate and incompletely understood mechanism. Although IL-1 beta is most often secreted in its active form, secretion of the uncleaved protein may be detectable under some biological conditions. IL-1 beta signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 alpha. IL-1 beta activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 beta play an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses to infection and injury. Elevated levels of IL-1 beta have been associated with many chronic inflammatory conditions IL-1 beta neutralizing antibodies potential therapeutic value.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.