
Thermo Fisher Scientific IL-1 beta Monoclonal Antibody (A7F9)
인간 IL-1 beta를 인식하는 Mouse IgG1 단클론 항체로, WB, ICC/IF, ELISA에 사용 가능. 단백질 A로 정제된 액상 형태이며, 2 mg/mL 농도. 염증성 사이토카인 연구 및 면역반응 분석에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| ELISA | 1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | A7F9 |
| Immunogen | Recombinant protein within Human IL-1 beta aa 117-269/269 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Purification | Protein A |
| Storage buffer | PBS, pH 7.4, with 0.1% BSA, 40% glycerol |
| Contains | 0.05% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_3091034 |
Product Specific Information
- Positive control: THP-1 treated with 80 nM TPA overnight, then treated with 100 ng/mL LPS for 6 hours and 300 ng/mL BFA for 3 hours whole cell lysate.
- Predicted band size: 31 kDa
- Subcellular Location: Extracellular exosome, Secreted, Lysosome, Cytosol
- Product MA5-50556 was used in an Indirect ELISA.
Target Information
Interleukin-1 beta (IL-1 beta) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. It is synthesized as a 31 kDa inactive pro-form that accumulates in the cytosol and requires inflammasome activation for cleavage into the active 17 kDa protein. IL-1 beta lacks a signal peptide for the classical ER/Golgi pathway and is secreted via an alternate mechanism. It signals through IL-1RI and IL-1RII receptors and is regulated by IL-1 Receptor Antagonist (IL-1RA). IL-1 beta plays a key role in innate host defense and is associated with chronic inflammatory conditions.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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