
Thermo Fisher Scientific Goat anti-Mouse IgM Cross-Adsorbed Secondary Antibody, DyLight 680
DyLight 680으로 결합된 염소 유래 항-마우스 IgM 2차 항체로, Western blot, IHC, ICC, Flow cytometry 등에 사용 가능. 인간 및 랫트에 대한 교차 반응 최소화. 고순도 항원 친화 크로마토그래피 정제. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:20,000 |
| Immunohistochemistry (IHC) | 1:50–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:500 |
| Flow Cytometry (Flow) | 1:50–1:200 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Mouse IgM |
| Conjugate | DyLight™ 680 |
| Excitation / Emission Max | 676 / 705 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 6.8–7.4, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2556734 |
| Target | IgM |
| Cross Adsorption | Against human and rat |
| Antibody Form | Whole Antibody |
Product Specific Information
This antibody is cross-adsorbed and exhibits minimal reactivity to human and rat.
Target Information
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins. They are useful for detection, sorting, or purification of mouse targets. Secondary antibodies provide versatility across detection systems (e.g., HRP, AP, fluorescence) and enhance sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody. These antibodies are typically generated by immunizing the host with pooled mouse immunoglobulins and further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to produce highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Available Conjugate Formats
- DyLight 488
- DyLight 550
- DyLight 594
- DyLight 650
- DyLight 755
- DyLight 800
- Custom conjugation available upon request
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