Thermo Fisher Scientific CD4 Monoclonal Antibody (RPA-T4), FITC, eBioscience

Applications

Immunohistochemistry (IHC)

• Tested Dilution: Not specified
• View 1 publication

Flow Cytometry (Flow)

• Tested Dilution: 5 µL (1 µg)/test
• View 48 publications

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 561, 647, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 496, 615, 661, 737, 805
• Brilliant Violet 421, 480, 605, 650, 711
• eFluor 450, 506
• Functional Grade
• NovaFluor Blue 510, 585, 690
• Pacific Orange
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Request custom conjugation

Product Specific Information

Description:
The RPA-T4 monoclonal antibody reacts with human CD4, a 59 kDa cell surface receptor expressed by most thymocytes, a subpopulation of mature T cells (T-helper cells), and at low levels on monocytes. CD4 serves as a receptor for the human immunodeficiency virus (HIV). RPA-T4 blocks HIV binding and mixed lymphocyte reaction. It recognizes a different epitope than the OKT4 antibody, and these antibodies do not cross-block each other.

Applications Reported:

• Flow cytometric analysis.

Applications Tested:

• Pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells.
• Recommended usage: 5 µL (1 µg) per test in a final volume of 100 µL.
• Cell number: 10⁵–10⁸ cells/test (empirically determined).

Optical Properties:

• Excitation: 488 nm
• Emission: 520 nm
• Laser: Blue Laser

Filtration:

• 0.2 µm post-manufacturing filtered.

Target Information

The CD4 antigen is involved in recognition of MHC class II molecules and functions as a co-receptor for HIV. It is primarily expressed on T-helper lymphocytes but also found on monocytes, macrophages, and dendritic cells. CD4 expression can be detected in thymus, lymph nodes, tonsils, spleen, and certain regions of the brain and gut. It plays a key role in initiating T-cell activation via association with the T-cell receptor complex and protein tyrosine kinase Lck. CD4 may also contribute to neuronal damage in infectious and immune-mediated CNS diseases. Multiple alternatively spliced transcripts have been identified (RefSeq, July 2017).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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