Thermo Fisher Scientific NSUN2 Polyclonal Antibody
NSUN2 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB, IHC(P), ICC/IF에 사용 가능. 인간, 마우스, 랫트에 반응하며, 고순도 친화 크로마토그래피 정제. PBS 및 50% 글리세롤 버퍼에 보관되며 -20°C에서 안정적 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human NSUN2 (Accession Q08J23), corresponding to amino acid residues P504–E554 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | -20°C |
| Shipping conditions | Wet ice |
| RRID | AB_2900309 |
Product Specific Information
This antibody detects endogenous levels of total NSUN2.
Target Information
NSUN2 encodes a methyltransferase that catalyzes the intron-dependent formation of 5-methylcytosine at C34 of tRNA-leu(CAA). Maturation of cytoplasmic tRNAs includes splicing of introns located one nucleotide 3′ from the anticodon in all intron-containing tRNA genes. In tRNA-leu(CAA), the first position of the anticodon (C34) is converted to 5-methylcytosine, a modification necessary to stabilize anticodon-codon pairing and ensure accurate mRNA translation. Mutations in NSUN2 are associated with autosomal recessive mental retardation type 5 (MRT5).
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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